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1·The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine was determined by dual-luciferase reporter assay.

通过双荧光素酶报告基因测定法测定了 IGFBP-3 对由三碘甲状腺素刺激的生长激素启动子活性的影响。

2·Methods The method of luciferase reporter gene was used.

方法采用荧光素酶报告基因方法。

3·The species, structure and applications of luciferase were researched.

文章就荧光素酶的种类、结构及应用进行了研究。

4·We addressed this question with the method of luciferase reporter gene.

我们用荧光素酶报告基因的方法，探讨了这个问题。

5·Luciferase reporter gene technique plays an important role in high throughput screening.

荧光素酶报告基因分析技术在药物的高通量筛选中起着重要的作用。

6·The cloning of luciferase from fireflies, which also took place in 1985, proved just as useful.

同年，克隆荧火虫的荧光素酶也取得成功，这个非常有用。

7·We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.

我们使用了依赖HCV IRES表达萤火虫荧光素酶的报告基因质粒。

8·Results A series of truncated NOS1 promoter luciferase reporter vectors were constructed and identified.

结果 构建并鉴定了系列截短的NOS1 启动子萤光素酶报告基因载体；

9·The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase.

相对荧光素酶活性法检测GR转录激活功能的变化。

10·Transcriptional activation activity of variant was evaluated through methods of relative activity of luciferase.

相对荧光素酶法检测转录激活活性。

荧光素酶

1·The investigators used genetically engineered E. coli cells to produce luciferase as a function of AHL concentration.

研究者用基因工程的大肠埃希菌细胞生产荧光素酶作为AHL浓度函数。

2·CONCLUSION: Luciferase reporter vectors regulated by HRE/CMV promoters were successfully constructed, which makes it possible to further investigate their potency of hypoxia regulation.

结论：成功构建了缺氧应答启动子的荧光素酶报告载体，为进一步研究其缺氧调控作用提供了条件。