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1·The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene.

通过转化绿色荧光蛋白基因质粒，对阿特拉津降解基因工程菌进行标记。

2·Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.

目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。

3·The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;

该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；