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1·The molecular weight was determined by tricine gel electrophoresis.

分子量通过三辛凝胶电泳确定。

2·Methods The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight was determined by tricine gel electro phoresis.

用离子交换柱层析法分离，用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量。