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1·Tube strain is first fixed, and after killing hybrid fungi, cooling, cutting strain volva, the strain is transplanted to new culture medium before a cotton cover is plugged.

其转接方法是：先固定试管菌种，经杀灭杂菌并冷却，划割菌种托(钩)菌块后转接新培养基，插盖棉塞。