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1·Fungal DNA was extracted from 43 samples of impacted low-melt AGAR by a 3-step extraction method and amplified by QPCR.

真菌dna从43份压缩空气采用三步提取法和QPCR法进行扩增来提取的。

2·As the QPCR technique continues to develop and improve, it will be more and more wildly applied in the field of environmental microorganisms.

随着该技术的进一步完善，其必将在环境微生物领域发挥越来越重要的作用。

3·Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.

目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。