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1·Methods Routine physiological and biochemical methods and real time PCR were used.

方法常规生理生化鉴定法和实时荧光pcr法。

2·Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.

目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。

3·Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.

目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。

4·To develop a fluorescent MGB probe real time PCR platform for detection the mutation of phenylalanine hydroxylase gene in patients with phenylketonuria.

探讨荧光mgb探针实时P CR技术检测经典型苯丙酮尿症的基因突变。

5·The facility provides technical services for the clients, which include Oligonucleotide Synthesis, Protein Sequencing, DNA Sequencing, Real Time PCR/Allele Detection.

该 机构为客户提供寡核苷酸合成、 蛋白质测序、 DNA测序、实时PCR和等位 基因检测等技术服务。

6·In the paper, the rapid and accurate methods for detection and identification of food borne pathogens were established using real time PCR, RT-real time PCR and DNA chip.

本文运用实时荧光pcr、实时荧光rt - PCR和DNA芯片技术建立了快速检测食源性致病菌的方法。

7·Each lab now has a real-time polymerase chain reaction (PCR) machine, and one lab has two machines.

每个实验室都有一台实时多聚酶链式反应机，有一个实验室有两台这样的机器。

8·All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.

应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。

9·Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).

最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。

10·Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.

利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。