全部

1·The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.

方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。

2·Using the organization to adherent culture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.

采用组织块贴壁培养法，成功地构建北京鸡成纤维靶细胞系。

3·Conclusion The improved method of whole bone marrow adherent. can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.

结论改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞，是一种比较理想的分离培养方法。