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1·The main applications of immobilized penicillin G acylase and lipase in industrial catalysis were introduced as well.

同时介绍了固定化青霉素酰化酶和固定化脂肪酶在工业催化领域的应用。

2·It was found that penicillin acylase activity of the immobilized cells was more stable than that of the intact cells.

发现固定化细胞的青霉素酰化酶比天然细胞的更稳定。

3·The genomic DNA was extracted from the selective cultures and the GL-7-ACA acylase gene was directly amplified by PCR technique.

从土壤的选择性培养液中提取微生物的DNA，通过PCR的方法直接扩增成功得到GL-7-ACA酰化酶基因。

4·S-2-phenylpropionic acid was obtained by hydrolysis of 2-phenylpropionic ester catalyzed by penicillin G acylase (PGA) in the micro-aqueous phase.

通过微水相优化拆分制备S-2-苯基丙酸的反应条件，确定了青霉素酰化酶拆分制备S-2-苯基丙酸的最佳工艺参数。

5·Acylase I was immobilized on Y-type molecular sieve by using 4 kinds of methods, namely, adsorption, adsorption-crosslink, coupling and coupling-diazotization.

以Y型分子筛为载体，分别采用吸附法、吸附-戊二醛交联法、偶联法和偶联-重氮法4种方法对氨基酰化酶i进行固定化。

6·Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor.

发酵过程中维持低葡萄糖水平可以限制细胞的生长速率，提高质粒稳定性和促进青霉素G酰化酶的合成。

7·Conclusions the usage of the new immobilized penicillin acylase reactor in the 6-apa production will result in a higher yield of 6-apa and lower loss of immobilized penicillin acylase.

结论6 APA生产中使用新的固定化青霉素酰化酶反应器将提高6 APA的收率，减少固定化青霉素酰化酶的损耗。

酰化酶

1·The main applications of immobilized penicillin G acylase and lipase in industrial catalysis were introduced as well.

同时介绍了固定化青霉素酰化酶和固定化脂肪酶在工业催化领域的应用。