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1·Suppression Subtractive Hybridization SSH is the most famous of subtraction methods Lukyanov et al.

抑止性消减杂交SSH是最有名地减法办法卢科亚诺夫等。

2·Method Subtractive hybridization technique was employed to screen down regulated genes of colorectal cancer.

方法采用减式杂交技术筛选大肠癌下调基因。

3·First, unincorporated adapters can randomly anneal to non-target cDNA molecules during subtractive hybridization.

起首，不法适配器能够随机退火对非目的基因分子在消减杂交。

4·Suppression subtractive hybridization has been widely used in medical research but less in plant science up to date.

差减抑制杂交技术在医学研究中应用较多，而在植物研究中的应用较少，近几年有所增加。

5·Differentially expressed gene librarys of the control and experimental groups were constructed by the method of suppression subtractive hybridization.

利用消减抑制杂交技术建立对照组与实验组差异表达基因文库。

6·Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).

目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。

7·Methods Improved PCR-based subtractive hybridization was used to clone the genes from apoptotic prostatic carcinoma cell line DU-145 cells induced by ATRA.

方法：应用全反式维甲酸诱导前列腺癌DU 145细胞凋亡，建立细胞凋亡模型，采用基于PCR的改良消减杂交技术克隆与凋亡相关的基因。

8·Subtractive hybridization is an approach allowing comparison of two DNA populations and isolation of fraction enriched in differentially distributed molecules.

消减杂交手艺是一种方法，许可两种DNA地生齿和孤立局部富集差别散布地分子。

9·Suppression subtractive hybridization(SSH), which is feasible and highly specific, is one of the most efficient methods for cloning differentially expressed genes.

抑制性消减杂交是一种简便易行、 特异性高的高效分离差异表达基因的方法。

10·Objective: To prepare polyclonal antibodies of MCPR1, which is a novel Mouse Cleft Palate Relate gene cloned by subtractive hybridization method at our department.

目的：MCPR 1是我室利用消减杂交技术克隆出来的新基因，本研究利用融合蛋白制备mcpr1的多克隆抗体。