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1·Transgenic animal is an ideal method to study the function of specific gene.

转基因动物的方法为研究基因的功能提供了一个理想的途径。

2·Gene transfer is a new and widely used technology in bioscience field. At present over 80 percent of transgenic animal models are constructed by means of microinjection.

转基因是近年来在生命科学领域广泛应用的新兴技术，目前80%以上的转基因动物模型是利用显微注射法构建完成的。

3·However, most of the traditional techniques for producing transgenic animal were quite complicate, high cost and little effective, which limits their extensive application.

但是传统的转基因动物技术大多操作复杂、成本高、效率低，从而限制了转基因技术的广泛应用。

4·Recently, with the development of transgenic technology, producing transgenic animal model has become an important way to study the expression regulation of eukaryotic genes.

近年来，随着转基因动物制备技术的日趋成熟与完善，建立转基因动物模型已经成为研究真核基因表达调控的一种重要手段。

5·The basic reliable methods available to produce transgenic animal such as pronuclear microinjection has limited application by low efficiency and mosaic integration into the genome.

由于生产转基因动物的基本方法如原核注射法等普遍存在外源基因整合的低效性和随机性等问题，因而在应用中受到很大制约。

6·Transgenic animal has been extended to various areas such as molecular biology, immunology, manufacture of pharmaceuticals and medicines, prologue breeding and organ transplantation.

转基因动物技术已广泛渗透于分子生物学、免疫学、生物制药、畜牧育种以及器官移植等研究领域中。

7·Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.

限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。

8·An Argentine laboratory announced that it had created the world's first transgenic cow, using human genes that will allow the animal to produce the equivalent of mothers' milk.

一个阿根廷的实验室宣布他们已经成功导入人类基因并培育出世界上第一头转基因奶牛。 导入的人类基因能够使奶牛产生含有人乳类似物的牛乳。

9·Since late 2005, however, three published studies by three different scientific teams and one unpublished study have actually detected transgenic plant DNA in animal tissues and milk.

2005年底以来，三个不同的科学研究小组发表的三篇研究报告以及一篇发表的研究报告事实上在动物组织和牛奶中检测到转基因植物的基因。

10·Transgenic technology is a important technology of modern life science. It has been widely used to study gene function and change genetic traits of animal.

转基因技术已成为现代生命科学中的一项重要技术，它不仅广泛地用于研究基因的功能，同时也用来快速改变生物的遗传性状。